Forensic transcriptome analysis using massively parallel sequencing

Abstract The application of transcriptome analyses in forensic genetics has experienced tremendous growth and development the past decade. earliest studies main applications were body fluid tissue identification, using targeted RNA transcripts a reverse transcription endpoint PCR method. A number markers have been identified for forensically most relevant fluids tissues method successfully used casework. introduction Massively Parallel Sequencing (MPS) opened up new perspectives opportunities to advance field. Contrary genomic DNA where two copies an autosomal segment are present cell, abundant species expressed high copy numbers. Even whole sequencing (RNA-Seq) postmortem material was shown be possible. This review gives overview on applications. methods cover as well MPS approaches. High resolution being applied fluid/ determination age stains donor, estimation post-mortem interval post mortem death investigations.